fluorescent assay Search Results


fluorescence polarization monoclonal immunoassay fpia  (Abbott Laboratories)
86
Abbott Laboratories fluorescence polarization monoclonal immunoassay fpia
Fluorescence Polarization Monoclonal Immunoassay Fpia, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zoe fluorescent cell imager  (Bio-Rad)
96
Bio-Rad zoe fluorescent cell imager
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Zoe Fluorescent Cell Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egfp  (Rockland Immunochemicals)
95
Rockland Immunochemicals egfp
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Egfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti green fluorescent protein  (Rockland Immunochemicals)
96
Rockland Immunochemicals anti green fluorescent protein
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Anti Green Fluorescent Protein, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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0260 blocking solution rockland  (Rockland Immunochemicals)
96
Rockland Immunochemicals 0260 blocking solution rockland
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
0260 Blocking Solution Rockland, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gfp primary antibodies  (Rockland Immunochemicals)
95
Rockland Immunochemicals anti gfp primary antibodies
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Anti Gfp Primary Antibodies, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein normalization  (Beijing Solarbio Science)
94
Beijing Solarbio Science protein normalization
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Protein Normalization, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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red fluorescent protein rfp  (Rockland Immunochemicals)
92
Rockland Immunochemicals red fluorescent protein rfp
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Red Fluorescent Protein Rfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal fitc conjugated anti gfp  (Rockland Immunochemicals)
95
Rockland Immunochemicals polyclonal fitc conjugated anti gfp
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Polyclonal Fitc Conjugated Anti Gfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horseradish peroxidase  (Rockland Immunochemicals)
95
Rockland Immunochemicals horseradish peroxidase
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Horseradish Peroxidase, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rfp  (Rockland Immunochemicals)
96
Rockland Immunochemicals rfp
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Rfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blocking solution  (Rockland Immunochemicals)
93
Rockland Immunochemicals blocking solution
Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained <t>in</t> <t>ZOE‐Fluorescent</t> cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).
Blocking Solution, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained in ZOE‐Fluorescent cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bioengineered AAV9 and Optimised Microdystrophin Vectors Augment Phenotypic Rescue in a Murine Model of Duchenne Muscular Dystrophy

doi: 10.1111/jcmm.71078

Figure Lengend Snippet: Validation of codon‐optimised microdystrophin transgene in vitro. Transduction of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys vectors in C2C12 cells (A), at an MOI: 1 × 10 5 and its quantification (B) are shown. Representative images were obtained in confocal microscope (AXR Nikon) with a 40X objective (Scale bar: 50 μm). Semi‐quantification was performed from images ( n ≥ 8 per group) obtained in ZOE‐Fluorescent cell imager. The data is represented as mean ± SD. Asterisk (*) represents statistical comparison of AAV9K51Q‐μDys and AAV9K51Q‐CoμDys treated conditions with respect to the cell control and Hash (#) refers to statistical comparison of AAV9K51Q‐CoμDys with respect to AAV9K51Q‐μDys. # p < 0.05, *** p < 0.001, **** p < 0.0001. For statistical comparison, a one‐way ANOVA was performed (GraphPad Prism 8.0.2 software).

Article Snippet: The images were obtained in the ZOE‐Fluorescent cell imager (Bio‐Rad, Hercules, CA, USA) and these images ( n ≥ 8/group) were further used for semi‐quantification analysis (ImageJ software).

Techniques: Biomarker Discovery, In Vitro, Transduction, Microscopy, Comparison, Control, Software